Recombinant Involucrin antibody

Catalog No. ABIN7655764

This Mouse Monoclonal antibody specifically detects Involucrin in IHC and IHC (fp). It exhibits reactivity toward Human, Dog, Pig, Gorilla and Owl Monkey.

Quick Overview for Recombinant Involucrin antibody (ABIN7655764)

Target: Involucrin (IVL)

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog, Pig, Gorilla, Owl Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RIVRN-827

Product Details anti-Involucrin Antibody

Purpose: Involucrin (Squamous Cell Terminal Differentiation Marker)(rIVRN/827)

Characteristics: Recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length Involucrin protein (IVRN)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background:

Synonyms: IVL

Gene Symbol: IVL

Tissue Expression: Epidermal cells|Epithelial cells|Eye

Molecular Weight: 66-170 kDa

Gene ID: 3713

UniProt: P07476