Cytokeratin 1 antibody (C-Term) (CF®405S)

Catalog No. ABIN7655830

This Mouse Monoclonal antibody specifically detects Cytokeratin 1 in IHC and IHC (fp). It exhibits reactivity toward Human and Rat.

Quick Overview for Cytokeratin 1 antibody (C-Term) (CF®405S) (ABIN7655830)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: LHK1

Product Details anti-Cytokeratin 1 Antibody (CF®405S)

Binding Specificity: C-Term

Purpose: Cytokeratin 1 (Suprabasal Keratinocyte Marker)(LHK1), CF405S conjugate

Characteristics: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10). Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A 13-amino acid peptide (VRFVSTSYSGVTR) from the C-terminus of human KRT1 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Skin or Cervix.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Keratin 1

Background:

Synonyms: 67 kDa Cytokeratin, CK1, Cytokeratin-1, EHK1, Epidermolytic hyperkeratosis 1, EPPK, K1, Keratin Type II Cytoskeletal 1, Keratin-1, KRT1, KRT1A, NEPPK, Type II cytoskeletal 1, Type-II keratin Kb1

Gene Symbol: KRT1

Tissue Expression: Epithelial cells

Molecular Weight: 67 kDa

Gene ID: 38748

UniProt: P04264