Recombinant Keratin 10 antibody (CF®647)

Catalog No. ABIN7655864

The Rabbit Monoclonal anti-Keratin 10 antibody has been validated for IHC and IHC (fp). It is suitable to detect Keratin 10 in samples from Human and Mouse.

Quick Overview for Recombinant Keratin 10 antibody (CF®647) (ABIN7655864)

Target: Keratin 10 (KRT10)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: KRT10-1990R

Product Details anti-Keratin 10 Antibody (CF®647)

Purpose: Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), CF647 conjugate

Characteristics: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length KRT10 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Keratin 10

Background:

Synonyms: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Gene Symbol: KRT10

Tissue Expression: Epidermal cells

Molecular Weight: 56.5 kDa

Gene ID: 3858

UniProt: P13645