KRT14 antibody (AA 351-472) (CF®640R)

Catalog No. ABIN7655917

This Mouse Monoclonal antibody specifically detects KRT14 in IHC, FACS and IHC (fp). It exhibits reactivity toward Human, Rat, Mouse and Pig.

Quick Overview for KRT14 antibody (AA 351-472) (CF®640R) (ABIN7655917)

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human, Rat, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: KRT14-2375

Product Details anti-KRT14 Antibody (CF®640R)

Binding Specificity: AA 351-472

Purpose: Cytokeratin 14 (KRT14) (Squamous Cell Marker)(KRT14/2375), CF640R conjugate

Characteristics: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human KRT14 fragment (around aa351-472)

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: A431 or HeLa cells. Skin or Squamous Cell Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Keratin 14

Background:

Synonyms: CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, k14, Keratin 14, Keratin Type I Cytoskeletal 14, Koebner, krt14, NFJ

Gene Symbol: KRT14

Tissue Expression: Epithelial cells

Molecular Weight: ~50 kDa

Gene ID: 3861

UniProt: P02533