KRT20 antibody (AA 196-323) (CF®568)

Catalog No. ABIN7656132

This anti-KRT20 antibody is a Mouse Monoclonal antibody detecting KRT20 in WB, IHC and IHC (fp). Suitable for Human.

Quick Overview for KRT20 antibody (AA 196-323) (CF®568) (ABIN7656132)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: KRT20-1991

Product Details anti-KRT20 Antibody (CF®568)

Binding Specificity: AA 196-323

Purpose: Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)(KRT20/1991), CF568 conjugate

Characteristics: This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HT29 cells. Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Keratin 20

Background:

Synonyms: CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20

Gene Symbol: KRT20

Tissue Expression: Goblet cells|Intestine

Molecular Weight: 46 kDa

Gene ID: 54474

UniProt: P35900