Keratin 7/17 antibody (CF®405S)

Catalog No. ABIN7656239

The Mouse Monoclonal anti-Keratin 7/17 antibody has been validated for IHC and IHC (fp). It is suitable to detect Keratin 7/17 in samples from Human and Cow.

Quick Overview for Keratin 7/17 antibody (CF®405S) (ABIN7656239)

Target: Keratin 7/17 (KRT7/17)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 7/17 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: C-46

Product Details anti-Keratin 7/17 Antibody (CF®405S)

Purpose: Cytokeratin 7/17(C-46), CF405S conjugate

Characteristics: Recognizes intermediate filament proteins (IFP's) of 55 kDa and 46 kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Alternative Name: Keratin 7/17

Background:

Synonyms: CK-7; K2C7; Keratin 55K Type II Cytoskeletal; Keratin Simple Epithelial Type 1 K7; Keratin Type II Cytoskeletal 7; Krt2-7; KRT7; Sarcolectin; SCL; Type II Mesothelial Keratin K7; Type-II Keratin Kb7; CK17; K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1

Gene Symbol: KRT7 & KRT17

Tissue Expression: Epithelial cells

Molecular Weight: 55 kDa (CK7), 46 kDa (CK17)

Gene ID: 3855, 3872

UniProt: P08729, Q04695