LSP1 antibody (AA 174-278) (Biotin)

Catalog No. ABIN7656440

The Mouse Monoclonal anti-LSP1 antibody has been validated for IHC and IHC (fp). It is suitable to detect LSP1 in samples from Human.

Quick Overview for LSP1 antibody (AA 174-278) (Biotin) (ABIN7656440)

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LSP1 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: LSP1-3025

Product Details anti-LSP1 Antibody (Biotin)

Binding Specificity: AA 174-278

Purpose: Lymphocyte Specific Protein 1 (LSP1) / pp52(LSP1/3025), Biotin conjugate

Characteristics: LSP1 is a hematopoietic-expressed gene that encodes an F-actin-binding, leukocyte-specific (including B and T lymphocytes, granulocytes and macrophages), phosphoprotein. However, mRNA splice variants that do not encode the lympho-specific protein are expressed from this gene in nonlymphoid cell lines as well (myocytes, stromal cells and fibroblasts), suggesting pp52 has a divergent role in signal transduction. The pp52 (LSP1) locus maps to human chromosome 11p15. 5, which is implicated in tumor-related chromosomal translocations found in chronic lymphocytic leukemia. The pp52 promoter contains key elements that control transcriptional activity including an initiator specifying the unique 5' terminus of pp52 mRNA, tandem pairs of Ets and SP1 motifs, and a single C/EBP motif. LSP1 binds the cytoskeleton and has been implicated in affecting cytoskeletal remodeling in a variety of leukocyte functions, including cell motility and chemotaxis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human LSP1 protein (around aa174-278)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Human tonsil, colon, spleen or Hodgkin lymphoma (IHC-P).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for LSP1

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Alternative Name: Lymphocyte Specific Protein 1

Background:

Synonyms: WP34, pp52, lymphocyte specific protein 1, leukocyte specific protein 1

Gene Symbol: LSP1

Tissue Expression: Leukocytes

Molecular Weight: 52 kDa

Gene ID: 4046

UniProt: P33241