MHC Class II antibody (CF®740)

Catalog No. ABIN7656451

This Mouse Monoclonal antibody specifically detects MHC Class II in FACS, WB, IP, Func and IF. It exhibits reactivity toward Human and Pig.

Quick Overview for MHC Class II antibody (CF®740) (ABIN7656451)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunofluorescence (IF)

Clone: SPV-L3

Product Details anti-MHC Class II Antibody (CF®740)

Purpose: MHC II (HLA-DQ)(SPV-L3), CF740 conjugate

Characteristics: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: T4-positive CTL clone HG-38

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Raji cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC-II

Background: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea

Molecular Weight: 60 kDa

Gene ID: 3117, 3118, 3119

UniProt: P01908, P01909, P01920