MHC Class II antibody (CF®594)

Catalog No. ABIN7656458

The Mouse Monoclonal anti-MHC Class II antibody has been validated for FACS, WB, Func, IF and RIA. It is suitable to detect MHC Class II in samples from Mouse.

Quick Overview for MHC Class II antibody (CF®594) (ABIN7656458)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), Immunofluorescence (IF), Radioimmunoassay (RIA)

Clone: MK-D6

Product Details anti-MHC Class II Antibody (CF®594)

Purpose: MHC II, Mouse(MK-D6), CF594 conjugate

Characteristics: The antibody is directed against Mouse major histocompatibility complex class II, I-A d. It does not react with I-Ak, I-Ab, I-As, I-Af or I-Aa. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Spleen cells from B10.D2 mice

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MPBMC or Thymus.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC-II

Background:

Synonyms: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea

Gene Symbol: H2-Ea-ps (Mouse)

Molecular Weight: kDa

Gene ID: 100504404

UniProt: P01904, P04224