Recombinant MVP antibody

Catalog No. ABIN7656499

This Rabbit Monoclonal antibody specifically detects MVP in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant MVP antibody (ABIN7656499)

Target: MVP (Major Vault Protein (MVP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: VP2897R

Product Details anti-MVP Antibody

Purpose: Major Vault Protein (MVP) (VP2897R)

Characteristics: This antibody recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human MVP protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: MCF-7 or HeLa cells. Breast tumors

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: Major Vault Protein

Background:

Synonyms: Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

Gene Symbol: MVP

Molecular Weight: 104-110 kDa

Gene ID: 9961

UniProt: Q14764