Recombinant Mammaglobin antibody (CF®405S)

Catalog No. ABIN7656521

The Rabbit Monoclonal anti-Mammaglobin antibody has been validated for IHC and IHC (fp). It is suitable to detect Mammaglobin in samples from Human.

Quick Overview for Recombinant Mammaglobin antibody (CF®405S) (ABIN7656521)

Target: Mammaglobin

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Mammaglobin antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MGB1-2123R

Product Details anti-Mammaglobin Antibody (CF®405S)

Purpose: Mammaglobin (SCGB2A2) (Breast Cancer Marker) (MGB1/2123R), CF405S conjugate

Characteristics: Mammaglobin is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Mammaglobin (SCGB2A2) protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.1-0.2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: SK-BR-3 cells or Breast Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Mammaglobin

Target: Mammaglobin

Background:

Synonyms: Mammaglobin-1 (MGB1), Mammaglobin-A, Secretoglobin family 2A member 2 (SCGB2A2), SG2A2, UGB2

Gene Symbol: SCGB2A2

Tissue Expression: Mammary gland

Molecular Weight: 18 kDa

Gene ID: 4250

UniProt: Q13296