Recombinant MMP3 antibody (AA 317-327) (CF®488A)

Catalog No. ABIN7656638

The Rabbit Monoclonal anti-MMP3 antibody has been validated for . It is suitable to detect MMP3 in samples from Human.

Quick Overview for Recombinant MMP3 antibody (AA 317-327) (CF®488A) (ABIN7656638)

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MMP3 antibody is conjugated to CF®488A

Application: Please inquire

Clone: MMP3-1994R

Product Details anti-MMP3 Antibody (CF®488A)

Binding Specificity: AA 317-327

Purpose: MMP3 (Marker of Metastasis and Rheumatoid Arthritis)(MMP3/1994R), CF488A conjugate

Characteristics: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 317-327) of human MMP3 protein

Isotype: IgG

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Human prostate or lung tissue. A-431 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MMP3

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Alternative Name: MMP3

Background:

Synonyms: CHDS6, Matrix metalloproteinase 3, Matrix metalloproteinase-3, MGC126102, MGC126103, MGC126104, MMP 3, MMP-3, MMP3, Proteoglycanase, SL-1, SL1, STMY, STMY1, STR1, Stromelysin 1, Stromelysin-1, Transin 1

Gene Symbol: MMP3

Molecular Weight: 57 kDa

Gene ID: 4314

UniProt: P08254