MCAM antibody (CF®740)

Catalog No. ABIN7656790

This Mouse Monoclonal antibody specifically detects MCAM in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for MCAM antibody (CF®740) (ABIN7656790)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MCAM-1101

Product Details anti-MCAM Antibody (CF®740)

Purpose: MCAM / MUC18 / Mucin 18 / CD146(MCAM/1101), CF740 conjugate

Characteristics: The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MCAM protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background:

Synonyms: Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1

Gene Symbol: MCAM

Molecular Weight: 130 kDa

Gene ID: 4162

UniProt: P43121