TIMP2 antibody (AA 48-220) (CF®594)

Catalog No. ABIN7656998

The Mouse Monoclonal anti-TIMP2 antibody has been validated for IHC and IHC (fp). It is suitable to detect TIMP2 in samples from Human.

Quick Overview for TIMP2 antibody (AA 48-220) (CF®594) (ABIN7656998)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is conjugated to CF®594

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: TIMP2-2044

Product Details anti-TIMP2 Antibody (CF®594)

Binding Specificity: AA 48-220

Purpose: TIMP2 (Tissue Inhibitor of Metalloproteinase 2) (TIMP2/2044), CF594 conjugate

Characteristics: This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TIMP2 protein fragment (aa 48-220)

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Background:

Synonyms: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, Tissue Inhibitor of Metalloproteinase 2

Gene Symbol: TIMP2

Molecular Weight: 21 kDa

Gene ID: 7077

UniProt: P16035

Pathways: cAMP Metabolic Process