Recombinant HLA-DP antibody (CF®640R)

Catalog No. ABIN7657052

The Rabbit Monoclonal anti-HLA-DP antibody has been validated for IF and FACS. It is suitable to detect HLA-DP in samples from Human.

Quick Overview for Recombinant HLA-DP antibody (CF®640R) (ABIN7657052)

Target: HLA-DP (MHC Class II HLA-DP (HLA-DP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HLA-DP antibody is conjugated to CF®640R

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HLA-DPB1-2862R

Product Details anti-HLA-DP Antibody (CF®640R)

Purpose: HLA-DP (MHC II)(HLA-DPB1/2862R), CF640R conjugate

Characteristics: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Isotype: IgG

Application Details

Application Notes: ELISA: For coating use Ab at 2-4 μg/mL, order Ab without BSA, Flow cytometry: 1-2 μg/million cells, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Raji cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for HLA-DP

Target: HLA-DP (MHC Class II HLA-DP (HLA-DP))

Alternative Name: HLA-DP

Background:

Synonyms: HLA class II histocompatibility antigen DP alpha 1 chain, HLA DP1A, HLA SB alpha chain, HLADP, HLADPA1, HLASB, HLASB histocompatibility type, Human MHC class II HLA SB alpha, Major histocompatibility complex class II DP alpha 1, MHC class II DP3 alpha, MHC class II DPA1, PLT1, Primed lymphocyte test 1

Gene Symbol: HLA-DPB1

Molecular Weight: 33-35 kDa

Gene ID: 3115

UniProt: P04440