MHC, Class I antibody (CF®740)

Catalog No. ABIN7657101

This anti-MHC, Class I antibody is a Mouse Monoclonal antibody detecting MHC, Class I in IF. Suitable for Mouse, Human and Non-Human Primate.

Quick Overview for MHC, Class I antibody (CF®740) (ABIN7657101)

Target: MHC, Class I

Reactivity: Mouse, Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is conjugated to CF®740

Application: Immunofluorescence (IF)

Clone: 246-B8-E7

Product Details anti-MHC, Class I Antibody (CF®740)

Purpose: MHC I(246-B8.E7), CF740 conjugate

Characteristics: Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human peripheral blood leukocytes

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: THP-1 and Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC-I

Background:

Synonyms: HLA-A, HLA-B, HLA-C, HLA class 1A, HLA class 1B, HLA class 1C, Major histocompatibility complex, class I, A B C, MHC class I HLA-A, MHC class I HLA-B, MHC class I HLA-C, MHC HLA-ABC

Gene Symbol: HLA-A

Molecular Weight: ~41 kDa

Gene ID: 3105

UniProt: P01889, P30443, P30499