Recombinant Moesin antibody (CF®640R)

Catalog No. ABIN7657219

This Mouse Monoclonal antibody specifically detects Moesin in WB, IHC, IF and IHC (fp). It exhibits reactivity toward Human and Rat.

Quick Overview for Recombinant Moesin antibody (CF®640R) (ABIN7657219)

Target: Moesin (MSN)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RMSN-492

Product Details anti-Moesin Antibody (CF®640R)

Purpose: Moesin (rMSN/492), CF640R conjugate

Characteristics: This antibody recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Moesin protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Western Blot 0.5-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HT-29, Jurkat, CH3LC, or HUVEC cells. Uterus, Placenta, Tonsil, Melanoma, Testicular Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: Moesin

Background:

Synonyms: Membrane-organizing extension spike protein, Moesin/anaplastic lymphoma kinase fusion protein, MSLK fusion

Gene Symbol: MSN

Molecular Weight: 78 kDa

Gene ID: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization