MYCN antibody (CF®405S)

Catalog No. ABIN7657682

The Mouse Monoclonal anti-MYCN antibody has been validated for . It is suitable to detect MYCN in samples from Human, Mouse and Rat.

Quick Overview for MYCN antibody (CF®405S) (ABIN7657682)

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYCN antibody is conjugated to CF®405S

Application: Please inquire

Clone: NMYC-1

Product Details anti-MYCN Antibody (CF®405S)

Purpose: n-Myc (Neuroblastoma Marker)(NMYC-1), CF405S conjugate

Characteristics: The v-Myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans, the family consists of five genes: c-Myc, N-Myc, R-Myc, L-Myc and B-Myc. Amplification of the N-Myc gene has been found in human neuroblastomas and cell lines. Its amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-Myc gene encodes a nuclear phosphoprotein that exhibits relatively short (30 min) half life in vivo. The prototype member of the family, c-Myc p67, binds DNA in a sequence-specific manner subsequent to dimerization with a second basic region helix-loop-helix leucine zipper motif protein, designated Max. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human n-Myc protein.

Isotype: IgG2a, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: 293T whole cell lysates or recombinant Human n-Myc protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MYCN

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Alternative Name: n-Myc

Background:

Synonyms: Class E basic helix-loop-helix protein 37 (bHLHe37), MODED, N-myc proto-oncogene protein, Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene, ODED, pp65/67, v myc (avian) myelocytomatosis viral related oncogene neuroblastoma derived

Gene Symbol: MYCN

Molecular Weight: 67 kDa

Gene ID: 4613

UniProt: P04198