NKX2-8 antibody (AA 10-123) (Biotin)

Catalog No. ABIN7657990

This Mouse Monoclonal antibody specifically detects NKX2-8 in WB. It exhibits reactivity toward Human.

Quick Overview for NKX2-8 antibody (AA 10-123) (Biotin) (ABIN7657990)

Target: NKX2-8 (NK2 Homeobox 8 (NKX2-8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX2-8 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Clone: NKX28-2548

Product Details anti-NKX2-8 Antibody (Biotin)

Binding Specificity: AA 10-123

Purpose: NKX2.8(NKX28/2548), Biotin conjugate

Characteristics: The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human recombinant NKX2.8 protein fragment (around aa 10-123)

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Flow cytometry: 1-2 μg/million cells, Western blot 0.5-1 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Human Liver, Kidney or Thyroid tissue (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for NKX2-8

Target: NKX2-8 (NK2 Homeobox 8 (NKX2-8))

Alternative Name: NKX2.8

Background:

Synonyms: Homeobox protein NK 2 homolog H, Homeobox protein NK-2 homolog H, Homeobox protein Nkx 2.8, Homeobox protein Nkx-2.8, NK 2 homolog 8, NK 2 homolog H, NK2 homeobox 8, NK2 transcription factor related, locus 8, Nkx2 9, Nkx2-8, NKX2.8, NKX28_HUMAN, NKX2G, NKX2H, OTTHUMP00000027959

Gene Symbol: NKX2-8

Molecular Weight: 26 kDa

Gene ID: 26257

UniProt: O15522