NKX2-8 antibody (C-Term)

Catalog No. ABIN2777459

The Rabbit Polyclonal anti-NKX2-8 antibody is suitable to detect NKX2-8 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog and Pig. It has been validated for WB.

Quick Overview for NKX2-8 antibody (C-Term) (ABIN2777459)

Target: NKX2-8 (NK2 Homeobox 8 (NKX2-8))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NKX2-8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NKX2-8 Antibody

Binding Specificity: C-Term

Purpose: NKX2-8 Antibody - C-terminal region

Sequence: GTAAAQEKCG APPAAACPLP GYPAFGPGSA LGLFPAYQHL ASPALVSWNW

Predicted Reactivity: Cow: 92%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NKX2-8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NKX2-8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106551

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NKX2-8

Target: NKX2-8 (NK2 Homeobox 8 (NKX2-8))

Alternative Name: NKX2-8

Background:

Background Information: A murine NKX2-8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. Nkx2.8 bound to the active AFP promoter, and antisense inhibition of Nkx2.8 mRNA translation selectively reduced expression of both the endogenous human AFP gene and transfected reporters containing the rat AFP promoter.

Gene Name: NK2 homeobox 8

Alternative Symbols: NKX2H, NKX2.8, Nkx2-9

Protein Name: Homeobox protein Nkx-2.8

Molecular Weight: 26kDa

Gene ID: 26257

NCBI Accession: NP_055175

UniProt: O15522