Nuclear Antigen antibody (CF®740)

Catalog No. ABIN7658073

The CF®740-conjugated Mouse Monoclonal anti-Nuclear Antigen antibody (Clone NM106) (ABIN7658073) specifically detects Nuclear Antigen in IF, FACS, IHC and IHC (fp). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Nuclear Antigen antibody (CF®740) (ABIN7658073)

Target: Nuclear Antigen

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®740

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NM106

Product Details

Purpose: Human Nuclear Antigen(NM106), CF740 conjugate

Characteristics: This MAb is an excellent marker for all nuclei in cells and tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Nuclei of HL60 cells

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Does not react with pig, others not known. Immunohistology formalin-paraffin 0.25-0.5 μg/mL. Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL

Comment:

Positive Control: All human cells. Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Nuclear Antigen

Background: Nuclear Antigen

Molecular Weight: Not Known