Nucleoli Marker antibody (CF®740)

Catalog No. ABIN7658111

The Mouse Monoclonal anti-Nucleoli Marker antibody is suitable to detect Nucleoli Marker in samples from Human. It has been validated for IF, FACS, IHC and IHC (fp).

Quick Overview for Nucleoli Marker antibody (CF®740) (ABIN7658111)

Target: Nucleoli Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®740

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NM95

Product Details

Purpose: Nucleolar / Nucleoli(NM95), CF740 conjugate

Characteristics: This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Nuclei of myeloid leukemia biopsy cells

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with mouse, rat or cow, others not known. Immunohistology formalin-paraffin 0.25-0.5 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunocytochemistry acetone-fixed cells 0.5-1.0 μg/mL. Immunofluorescence 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Any human cells. Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Nucleoli Marker

Alternative Name: Nucleoli

Background: Not Known

Molecular Weight: Not Known