NME2 antibody (CF®405S)

Catalog No. ABIN7658185

This Mouse Monoclonal antibody specifically detects NME2 in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for NME2 antibody (CF®405S) (ABIN7658185)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NME2 antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CPTC-NME2-2

Product Details anti-NME2 Antibody (CF®405S)

Purpose: NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis)(CPTC-NME2-2), CF405S conjugate

Characteristics: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human NME2 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: PC3, A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for NME2

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Alternative Name: NDPK-B

Background:

Synonyms: C myc purine binding transcription factor PUF, C-myc purine-binding transcription factor PUF, epididymis secretory sperm binding protein Li 155an, HEL-S-155an, Histidine protein kinase NDKB, , NDP kinase B, NDPKB, nm23-H2, NM23B, NME/NM23 nucleoside diphosphate kinase 2, nme2, Nucleoside diphosphate kinase B,

Gene Symbol: NME2

Molecular Weight: 17 kDa

Gene ID: 4831

UniProt: P22392