Recombinant pan Keratin antibody (basic) (CF®647)

Catalog No. ABIN7658408

The Rabbit Monoclonal anti-pan Keratin antibody has been validated for IF, IHC and IHC (fp). It is suitable to detect pan Keratin in samples from Human, Rat and Mouse.

Quick Overview for Recombinant pan Keratin antibody (basic) (CF®647) (ABIN7658408)

Target: pan Keratin (panKRT)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to CF®647

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: KRTH-2147R

Product Details anti-pan Keratin Antibody (CF®647)

Binding Specificity: basic

Purpose: Cytokeratin, Basic (Type II or HMW)(KRTH/2147R), CF647 conjugate

Characteristics: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Solubilized keratin extract from human stratum corneum

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Keratin

Background:

Synonyms: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Gene Symbol: KRT76

Tissue Expression: Epidermal cells|Epithelial cells

Molecular Weight: 50-67 kDa

Gene ID: 51350

UniProt: Q01546