pan Keratin antibody (CF®740)

Catalog No. ABIN7658422

This Mouse Monoclonal antibody specifically detects pan Keratin in IF, FACS, WB, IHC and IHC (fp). It exhibits reactivity toward Human, Rat, Mouse, Cow, Pig, Frog, Guinea Pig, Goat and Marmoset.

Quick Overview for pan Keratin antibody (CF®740) (ABIN7658422)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Pig, Frog, Guinea Pig, Goat, Marmoset

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to CF®740

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: C11

Product Details anti-pan Keratin Antibody (CF®740)

Purpose: Cytokeratin, multi(C11), CF740 conjugate

Characteristics: This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Keratin-enriched preparation from cultured human A431

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1.0 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A431 cells, Skin, Colon carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Keratin, multi

Background:

Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Gene Symbol: KRT4

Tissue Expression: Epidermal cells|Epithelial cells

Molecular Weight: Multiple

Gene ID: 3851