pan Keratin antibody (acidic) (CF®488A)

Catalog No. ABIN7658439

This anti-pan Keratin antibody is a Mouse Monoclonal antibody detecting pan Keratin in . Suitable for Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit, Chicken and Turtle.

Quick Overview for pan Keratin antibody (acidic) (CF®488A) (ABIN7658439)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit, Chicken, Turtle

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to CF®488A

Application: Please inquire

Clone: AE-1

Product Details anti-pan Keratin Antibody (CF®488A)

Binding Specificity: acidic

Purpose: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)(AE-1), CF488A conjugate

Characteristics: This MAb recognizes the 56. 5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e. g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human epidermal keratin

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Skin or squamous cell carcinoma (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Keratin

Background:

Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Gene Symbol: KRT77

Tissue Expression: Epidermal cells|Epithelial cells

Molecular Weight: 40-56.5 kDa

Gene ID: 374454

UniProt: Q7Z794