PDLIM1 antibody

Catalog No. ABIN7658519

The Mouse Monoclonal anti-PDLIM1 antibody has been validated for IHC and IHC (fp). It is suitable to detect PDLIM1 in samples from Human.

Quick Overview for PDLIM1 antibody (ABIN7658519)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDLIM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CPTC-PDLIM1-1

Product Details anti-PDLIM1 Antibody

Purpose: PDZ and LIM Domain 1(CPTC-PDLIM1-1)

Characteristics: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88 % sequence similarity with its rat counterpart, suggesting a conserved function between species. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length PDLIM1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Alternative Name: PDZ and LIM Domain 1

Background:

Synonyms: C terminal LIM domain protein 1, carboxy terminal LIM domain protein 1, CLIM1, CLP36, Elfin, Enigma antibody, Enigma homolog antibody, hCLIM1, PDLIM1, PDZ and LIM domain 1

Gene Symbol: PDLIM1

Molecular Weight: 36 kDa

Gene ID: 9124

UniProt: O00151