Recombinant Phosphotyrosine antibody (Phosphospecific)

Catalog No. ABIN7658616

The Rabbit Monoclonal anti-Phosphotyrosine antibody (Clone PY2870R) (ABIN7658616) specifically detects Phosphotyrosine. The antibody is reactive with Various Species samples.

Quick Overview for Recombinant Phosphotyrosine antibody (Phosphospecific) (ABIN7658616)

Target: Phosphotyrosine

Antibody Type: Recombinant Antibody

Reactivity: Various Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Please inquire

Clone: PY2870R

Product Details anti-Phosphotyrosine Antibody

Binding Specificity: Phosphospecific

Purpose: Phosphotyrosine (P-Tyr)(PY2870R)

Characteristics: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human phosphotyrosine protein

Isotype: IgG

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: MCF-7, MDA-231, T47-D cells. Breast carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: P-Tyr

Molecular Weight: Depends upon the phosphorylated target