CD31 antibody (AA 625-738) (CF®568)

Catalog No. ABIN7658662

This Mouse Monoclonal antibody specifically detects CD31 in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for CD31 antibody (AA 625-738) (CF®568) (ABIN7658662)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PECAM1-3528

Product Details anti-CD31 Antibody (CF®568)

Binding Specificity: AA 625-738

Purpose: CD31 / PECAM-1 (Endothelial Cell Marker)(PECAM1/3528), CF568 conjugate

Characteristics: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 625-738) of human CD31 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Jurkat cells. Tonsil or Angiosarcoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background:

Synonyms: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA

Gene Symbol: PECAM1

Tissue Expression: Endothelial cells

Molecular Weight: ~100 kDa (endothelium) and ~130 kDa (platelets)

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization