Podoplanin antibody (AA 24-126) (CF®740)

Catalog No. ABIN7658756

The Mouse Monoclonal anti-Podoplanin antibody has been validated for IHC and IHC (fp). It is suitable to detect Podoplanin in samples from Human.

Quick Overview for Podoplanin antibody (AA 24-126) (CF®740) (ABIN7658756)

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PDPN-1433

Product Details anti-Podoplanin Antibody (CF®740)

Binding Specificity: AA 24-126

Purpose: Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) (PDPN/1433), CF740 conjugate

Characteristics: This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi'sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Podoplanin (PDPN) protein fragment (aa24-126)

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa Cells. Angiosarcoma or Mesothelioma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin

Background:

Synonyms: Aggrus, Glycoprotein 36 KD, Glycoprotein 36, gp36, GP38, GP40, HT1A1, hT1alpha1, hT1alpha2, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein T1A 2, OTS8, PA2.26, Pdpn, Podoplanin, PSEC0003, PSEC0025, T1-alpha, T1A, TI1A, TIA2

Gene Symbol: PDPN

Tissue Expression: Lymphatics

Molecular Weight: 38-43 kDa

Gene ID: 10630

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport