PD-1 antibody (CF®405S)

Catalog No. ABIN7658948

The Rat Monoclonal anti-PD-1 antibody has been validated for FACS and Func. It is suitable to detect PD-1 in samples from Mouse.

Quick Overview for PD-1 antibody (CF®405S) (ABIN7658948)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PD-1 antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: RMP1-14

Product Details anti-PD-1 Antibody (CF®405S)

Purpose: PD1, Mouse(RMP1-14), CF405S conjugate

Characteristics: PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally, Pdcd1mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: PDCD1 transfected BHK cells

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Spleen, Thymus or Lymph Node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD1

Background:

Synonyms: CD279, CD279 antigen, PD1, PDCD1, Programmed cell death 1, Programmed cell death protein 1, Systemic lupus erythematosus susceptibility 2 (SLEB2)

Tissue Expression: B-cells|Myeloid cells|T-cells

Molecular Weight: ~50-55 kDa

Gene ID: 18566

UniProt: Q02242

Pathways: Cancer Immune Checkpoints