Recombinant PD-1 antibody (CF®740)

Catalog No. ABIN7658964

This anti-PD-1 antibody is a Rabbit Monoclonal antibody detecting PD-1 in IHC and IHC (fp). Suitable for Human.

Quick Overview for Recombinant PD-1 antibody (CF®740) (ABIN7658964)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PD-1 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PDCD1-1410R

Product Details anti-PD-1 Antibody (CF®740)

Purpose: PDCD1 / PD1 / CD279 (Programmed Cell Death 1) (PDCD1/1410R), CF740 conjugate

Characteristics: PD-1 (also known as Programmed Death 1, CD279, and PDCD1) is a negative regulatory T-cell surface receptor. PD-1 is a Type I transmembrane protein expressed on the plasma membrane of T-cells. It is a T-cell checkpoint protein, involved in preventing autoimmunity. Because binding of PD-1 by its ligands PD-L1 or PD-L2 has a suppressive effect on the immune system, inhibition of the PD-1/PD-L1 pathway is a major strategy of immune-oncology therapies. PD-L1 is upregulated in certain cancers, and blocking PD-1 or PD-L1 can inhibit cancer growth. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human recombinant PDCD1 protein fragment

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: TY cells or Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: CD279

Background:

Synonyms: CD279, hPD-1, hSLE1, PD1, PDCD1, Programmed Cell Death Protein 1, Protein PD-1, SLEB2, Systemic lupus erythematosus susceptibility 2

Gene Symbol: PDCD1

Tissue Expression: B-cells|Myeloid cells|T-cells

Molecular Weight: 55 kDa

Gene ID: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints