PDCD1LG2 antibody (AA 27-220) (CF®647)

Catalog No. ABIN7658992

This Mouse Monoclonal antibody specifically detects PDCD1LG2 in FACS. It exhibits reactivity toward Human.

Quick Overview for PDCD1LG2 antibody (AA 27-220) (CF®647) (ABIN7658992)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDCD1LG2 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS)

Clone: PDL2-1850

Product Details anti-PDCD1LG2 Antibody (CF®647)

Binding Specificity: AA 27-220

Purpose: PD-L2 / PDCD1LG2 / CD273(PDL2/1850), CF647 conjugate

Characteristics: Recognizes a protein of about 31 kDa, which is identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 27-220) of human PD-L2 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 0.5-1 μg/million cells, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Placenta or Pancreas. Thyroid Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: CD273

Background:

Synonyms: B7 dendritic cell molecule, B7-DC, B7DC, Btdc, Butyrophilin B7DC, CD273, PD-L2, PDCD1L2, PDCD1LG2, PDL2, Programmed death ligand 2

Gene Symbol: PDCD1LG2

Molecular Weight: 31 kDa

Gene ID: 80380