RBP1 antibody (CF®740)

Catalog No. ABIN7659633

This anti-RBP1 antibody is a Mouse Monoclonal antibody detecting RBP1 in IHC and IHC (fp). Suitable for Human, Mouse, Rat, Rabbit, Monkey, Goat and Chimpanzee.

Quick Overview for RBP1 antibody (CF®740) (ABIN7659633)

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Reactivity: Human, Mouse, Rat, Rabbit, Monkey, Goat, Chimpanzee

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBP1 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: G4E4

Product Details anti-RBP1 Antibody (CF®740)

Purpose: Retinol Binding Protein-1(G4E4), CF740 conjugate

Characteristics: This MAb recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Retinol binding protein-1 purified from human plasma

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Hepatic or ovarian carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for RBP1

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Alternative Name: Retinol Binding Protein 1

Background:

Synonyms: Cellular retinol-binding protein I, CRBP1, CRBP2, RBP1, RBP2, RBP4, RBPC, Retinol binding protein 1, Retinol binding protein 1 cellular, Retinol binding protein 2 cellular, Retinol binding protein 4 plasma

Gene Symbol: RBP1

Molecular Weight: 21-25 kDa

Gene ID: 5947, 5948, 5950

UniProt: P02753, P09455, P50120