RNA Polymerase II antibody (pSer5) (CF®594)

Catalog No. ABIN7659657

This anti-RNA Polymerase II antibody is a Mouse Monoclonal antibody detecting RNA Polymerase II in WB, IHC (fp) and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for RNA Polymerase II antibody (pSer5) (CF®594) (ABIN7659657)

Target: RNA Polymerase II

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNA Polymerase II antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (IHC)

Clone: CTD4H8

Product Details anti-RNA Polymerase II Antibody (CF®594)

Binding Specificity: pSer5

Purpose: RNA Polymerase II(CTD4H8), CF594 conjugate

Characteristics: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1, 840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for RNA Polymerase II

Target: RNA Polymerase II

Background:

Synonyms: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit, DNA-directed RNA polymerase II subunit RPB1, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa, RNA polymerase II subunit B1, RPBh1, RpIILS, RPO2, RPOL2

Gene Symbol: POLR2A

Molecular Weight: 192-253 kDa

Gene ID: 5430

UniProt: P24928