s100a4 antibody (CF®740)

Catalog No. ABIN7659707

The Mouse Monoclonal anti-s100a4 antibody has been validated for WB, IHC, IF, FACS and IHC (fp). It is suitable to detect s100a4 in samples from Human and Mouse.

Quick Overview for s100a4 antibody (CF®740) (ABIN7659707)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This s100a4 antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: S100A4-1482

Product Details anti-s100a4 Antibody (CF®740)

Purpose: S100A4 (Marker of Tumor Metastasis) (S100A4/1482), CF740 conjugate

Characteristics: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, i±6i²4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human S100A4 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, A549 or A375 Cells. Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4

Background:

Synonyms: 18A2|42A|CAPL|Malignant transformation suppression 1 (MTS1)|Metastasin|P9KA|PEL98|Placental calcium-binding protein|S100 calcium-binding protein A4|S100A4

Gene Symbol: S100A4

Molecular Weight: 10-12 kDa

Gene ID: 6275

UniProt: P26447

Pathways: S100 Proteins