L-Selectin antibody (CF®568)

Catalog No. ABIN7659749

The Mouse Monoclonal anti-L-Selectin antibody has been validated for FACS, WB, IHC, IP, ELISA, Func and IHC (fp). It is suitable to detect L-Selectin in samples from Human.

Quick Overview for L-Selectin antibody (CF®568) (ABIN7659749)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Functional Studies (Func), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: LAM1-116

Product Details anti-L-Selectin Antibody (CF®568)

Purpose: CD62L (L-Selectin)(LAM1-116), CF568 conjugate

Characteristics: Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: HPBL or Jurkat whole cell lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L

Background:

Synonyms: A.11, AI528707, CD62 antigen ligand, CD62L, gp90-MEL, IgA nephropathy, susceptibility to, included, L Selectin, L-selectin, LAM1, LECAM1, LEU8, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lnhr, LSEL, Ly-22, LYAM1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, Pln homing receptor, PLNHR, SELL, TQ1

Gene Symbol: SELL

Tissue Expression: Eosinophils|Lymphocytes|Monocytes/macrophages|Neutrophils

Molecular Weight: 65-75 kDa

Gene ID: 6402

UniProt: P14151