SHBG antibody (CF®740)

Catalog No. ABIN7659881

The Mouse Monoclonal anti-SHBG antibody has been validated for IHC and IHC (fp). It is suitable to detect SHBG in samples from Human.

Quick Overview for SHBG antibody (CF®740) (ABIN7659881)

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHBG antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: SHBG-245

Product Details anti-SHBG Antibody (CF®740)

Purpose: SHBG (Sex Hormone Binding Globulin)(SHBG/245), CF740 conjugate

Characteristics: Recognizes a protein of 45 kDa, identified as SHBG. It functions as an androgen transport protein, but may also be involved in receptor-mediated processes. Each dimer binds one molecule of steroid. It is specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. SHBG regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human SHBG protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunofluorescence 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Liver, Placenta or Testis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for SHBG

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Alternative Name: SHBG

Background:

Synonyms: Androgen binding protein (ABP), Sex hormone-binding globulin (SHBG), Sex steroid binding protein (SBP), Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone binding beta globulin, Testosterone-Estradiol-Binding Globulin (TEBG)

Gene Symbol: SHBG

Tissue Expression: Testis

Molecular Weight: 45 kDa

Gene ID: 6462

UniProt: P04278

Pathways: Hormone Transport