Sialoadhesin/CD169 antibody (N-Term) (CF®488A)

Catalog No. ABIN7659887

The Mouse Monoclonal anti-Sialoadhesin/CD169 antibody has been validated for FACS, WB, IHC, Func and IHC (fp). It is suitable to detect Sialoadhesin/CD169 in samples from Human.

Quick Overview for Sialoadhesin/CD169 antibody (N-Term) (CF®488A) (ABIN7659887)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Sialoadhesin/CD169 antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: HSn 7D2

Product Details anti-Sialoadhesin/CD169 Antibody (CF®488A)

Binding Specificity: N-Term

Purpose: SIGLEC1 / CD169 / Sialoadhesin(HSn 7D2), CF488A conjugate

Characteristics: Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1, 709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Human spleen, human peripheral blood monocytes or MCF7 cell.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Alternative Name: CD169

Background:

Synonyms: CD169, CD169 antigen, Sialic acid binding immunoglobulin like lectin 1, Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1, SN

Gene Symbol: SIGLEC1

Molecular Weight: 185 kDa

Gene ID: 6614