Spastin antibody (CF®740)

Catalog No. ABIN7660080

This anti-Spastin antibody is a Mouse Monoclonal antibody detecting Spastin in WB. Suitable for Mouse, Rat and Human.

Quick Overview for Spastin antibody (CF®740) (ABIN7660080)

Target: Spastin (SPAST)

Reactivity: Mouse, Rat, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Spastin antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: Sp 6C6

Product Details anti-Spastin Antibody (CF®740)

Purpose: Spastin(Sp 6C6), CF740 conjugate

Characteristics: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Spastin protein.

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: HeLa cells. K-562 or Raji cell lysates.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Spastin

Target: Spastin (SPAST)

Alternative Name: Spastin

Background:

Synonyms: ADPSP, FSP2, Spast, Spatin, SPG4

Gene Symbol: SPAST

Molecular Weight: 52 kDa

Gene ID: 6683

Pathways: Microtubule Dynamics, M Phase, Regulation of Cell Size