Spastin antibody

Catalog No. ABIN6940629

Product Details anti-Spastin Antibody

Target: Spastin (SPAST)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Spastin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human Spastin protein.

Clone: Sp 3G11-1

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Rat brain extract.

Known Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Spastin

Target: Spastin (SPAST)

Alternative Name: SPAST (SPAST Products)

Synonyms: CG5977 antibody, D-spastin antibody, Dmel\\CG5977 antibody, Dspastin antibody, Spas antibody, zgc:85952 antibody, SPAST antibody, spg4 antibody, LOC100224472 antibody, ADPSP antibody, FSP2 antibody, SPG4 antibody, Spg4 antibody, mKIAA1083 antibody, spastin antibody, spastin S homeolog antibody, spas antibody, spast antibody, SPAST antibody, Spast antibody, spast.S antibody

Background: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.

Molecular Weight: 52kDa

Gene ID: 6683

Pathways: Microtubule Dynamics, M Phase, Regulation of Cell Size