SPTBN1 antibody

Catalog No. ABIN7660160

The Mouse Monoclonal anti-SPTBN1 antibody has been validated for WB and IF. It is suitable to detect SPTBN1 in samples from Human, Mouse and Rat.

Quick Overview for SPTBN1 antibody (ABIN7660160)

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTBN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: RG-26

Product Details anti-SPTBN1 Antibody

Purpose: Spectrin beta I (SPTB)(RG/26)

Characteristics: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Red cell membranes

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for SPTBN1

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Alternative Name: Spectrin beta I

Background:

Synonyms: beta-I spectrin, EL3, erythrocyte, HS2, SPTB1, HSPTB1, membrane cytoskeletal protein, spectrin beta (erythrocytic)

Gene Symbol: SPTB

Molecular Weight: 246 kDa

Gene ID: 6710

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Protein targeting to Nucleus