SPTBN1 antibody (AA 620-700)

Catalog No. ABIN7224444

The Rabbit Polyclonal anti-SPTBN1 antibody (ABIN7224444) specifically detects SPTBN1 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SPTBN1 antibody (AA 620-700) (ABIN7224444)

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTBN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SPTBN1 Antibody

Binding Specificity: AA 620-700

Purpose: Rabbit Anti-Spectrin β II Polyclonal Antibody

Specificity: Spectrin β II Polyclonal Antibody detects endogenous levels of Spectrin β II protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Spectrin beta II at AA range: 620-700

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPTBN1

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Alternative Name: Spectrin beta II

Background: SPTBN1, SPTB2, Spectrin beta chain, non-erythrocytic 1, Beta-II spectrin, Fodrin beta chain, Spectrin, non-erythroid beta chain 1Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTBN1 (spectrin beta, non-erythrocytic 1) is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for SPTBN1.

Molecular Weight: 275kD

Gene ID: 6711

UniProt: Q01082

Pathways: Regulation of Actin Filament Polymerization, Protein targeting to Nucleus