Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (CF®405S)

Catalog No. ABIN7660163

The Rabbit Monoclonal anti- antibody has been validated for WB, FACS and IF. It is suitable to detect in samples from Human.

Quick Overview for Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (CF®405S) (ABIN7660163)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: CF®405S

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPTBN2-2887R

Product Details

Binding Specificity: AA 356-475

Purpose: Spectrin beta III (SPTBN2)(SPTBN2/2887R), CF405S conjugate

Characteristics: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human SPTBN2 fragment (aa356-475)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa cells. Pancreas or Liver.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Alternative Name: Spectrin Beta-III

Background:

Synonyms: Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

Gene Symbol: SPTBN2

Molecular Weight: 246 kDa

Gene ID: 6712

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis