Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (CF®405S)

Catalog No. ABIN7660164

This anti- antibody is a Rabbit Monoclonal antibody detecting in WB, FACS, IHC and IHC (fp). Suitable for Human.

Quick Overview for Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (CF®405S) (ABIN7660164)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: CF®405S

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: SPTBN2-2894R

Product Details

Binding Specificity: AA 356-475

Purpose: Spectrin beta III (SPTBN2) (SPTBN2/2894R), CF405S conjugate

Characteristics: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta I (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. Spectrin beta III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human SPTBN2 fragment (aa356-475)

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa cells. Pancreas or Liver.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Alternative Name: Spectrin Beta-III

Background:

Synonyms: Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

Gene Symbol: SPTBN2

Molecular Weight: 246 kDa

Gene ID: 6712

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis