SCCA1 antibody (CF®640R)

Catalog No. ABIN7660237

This Mouse Monoclonal antibody specifically detects SCCA1 in WB. It exhibits reactivity toward Human.

Quick Overview for SCCA1 antibody (CF®640R) (ABIN7660237)

Target: SCCA1 (Squamous Cell Carcinoma Antigen 1 (SCCA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCCA1 antibody is conjugated to CF®640R

Application: Western Blotting (WB)

Clone: CPTC-SERPINB3-2

Product Details anti-SCCA1 Antibody (CF®640R)

Purpose: Squamous Cell Carcinoma Antigen 1(CPTC-SERPINB3-2), CF640R conjugate

Characteristics: Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine proteases, such as neutrophil elastase. Serpins are family of serine protease inhibitors, contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive. Aberrant expression of serpin family members can contribute to a number of conditions, including emphysema (-1 antitrypsin deficiency), fatal bleeding (elastase to Thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers). SCCA1 expression has been demonstrated to promote oncogenic transformation and epithelial-mesenchymal transition (EMT) in mammary epithelial cells, and its silencing in breast cancer cells has been shown to halt their proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length SERPINB3 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: U-2197, HaCaT, Squamous cells. Expressed in some hepatocellular carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for SCCA1

Target: SCCA1 (Squamous Cell Carcinoma Antigen 1 (SCCA1))

Alternative Name: Squamous Cell Carcinoma Antigen 1

Background:

Synonyms: HsT1196, Protein T4 A, Protein T4-A, SCC, SCCA-PD, SCCA1, SCCAPD, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3, Serpin B3, SERPINB3, Squamous cell carcinoma antigen 1, T4 A, T4-A

Gene Symbol: SERPINB3

Molecular Weight: 39 kDa

Gene ID: 6317

UniProt: P29508