CD37 antibody (CF®740)

Catalog No. ABIN7660685

The Mouse Monoclonal anti-CD37 antibody has been validated for FACS, IF, IHC and IHC (fp). It is suitable to detect CD37 in samples from Human.

Quick Overview for CD37 antibody (CF®740) (ABIN7660685)

Target: CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD37 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: IPO-24

Product Details anti-CD37 Antibody (CF®740)

Purpose: CD37(IPO-24), CF740 conjugate

Characteristics: Recognizes a protein of 33-55 kDa, identified as CD37 (Workshop V, Code CD37.7). CD37 is strongly expressed on normal and neoplastic mature (sIg ) B-lymphocytes. In B-cell ontogeny, CD37 appears after the pre-B-cell stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells.1 CD37 is also present, at low densities, on resting and activated T cells, neutrophils, monocytes, and some myelomonocytic leukemia cells. It is absent from platelets, erythrocytes. CD37 is a member of a family of tetraspan transmembrane proteins, including CD9, CD53, CD63, CD81, and CD82. It associates other tetraspan transmembrane proteins and MHC class II molecules to form a large complex at the surface of B cells and play a role in signal transduction. CD37 is a valuable and stable marker for peripheral mature B-cells and corresponding malignancies like B-cell chronic lymphocytic leukemia (B-CLL), hairy cell leukemia (HCL), and all types of B-cell non-Hodgkin'' lymphoma (B-NHL).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Spleen cells of a patient with hairy cell leukemia

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Daudi, Raji, Namalva, Ramos cells and human lymphocytes. Human tonsil and lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD37

Target: CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

Alternative Name: CD37

Background:

Synonyms: Cell differentiation antigen 37, GP52 40, Tetraspanin-26, Tspan-26, TSPAN26

Gene Symbol: CD37

Tissue Expression: Lymphocytes|Myeloid cells

Molecular Weight: 33-55 kDa

Gene ID: 951

UniProt: P11049

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response