TGF-beta antibody (CF®740)

Catalog No. ABIN7660691

The Mouse Monoclonal anti-TGF-beta antibody has been validated for Func, IHC and IHC (fp). It is suitable to detect TGF-beta in samples from Human, Mouse, Rat, Cow, Dog, Hamster and Monkey.

Quick Overview for TGF-beta antibody (CF®740) (ABIN7660691)

Target: TGF-beta (TGFb)

Reactivity: Human, Mouse, Rat, Cow, Dog, Hamster, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGF-beta antibody is conjugated to CF®740

Application: Functional Studies (Func), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 1D11-16-8

Product Details anti-TGF-beta Antibody (CF®740)

Purpose: TGF-beta(1D11.16.8), CF740 conjugate

Characteristics: This MAb recognizes TGF beta 1, 2 and 3. Three TGF-betas have been identified in mammals. TGF-beta1, TGF-beta2 and TGF-beta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGF-beta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGF-beta3 protein has approximately 80 % identity to the mature region of both TGF-beta1 and TGF-beta2. However, the NH2 terminals or precursor regions of their molecules share only 27 % sequence identity. TGF-beta's inhibit the growth of epithelial cells and stimulate the growth of mesenchymal cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Bovine TGF-beta2 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Cytokine neutralization or functional assay, order Ab without BSA/azide. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Salivary gland carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TGF-beta

Target: TGF-beta (TGFb)

Alternative Name: TGF-Beta

Background:

Synonyms: GP40, Leu9, p41, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp40, TP41

Gene Symbol: TGFB1

Molecular Weight: ~13 kDa

Gene ID: 7040

Pathways: EGFR Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus