XBP1 antibody (AA 51-150)

Catalog No. ABIN732728

The Rabbit Polyclonal anti-XBP1 antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect XBP1 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for XBP1 antibody (AA 51-150) (ABIN732728)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-XBP1 Antibody

Binding Specificity: AA 51-150

Specificity: XBP-1 antibody will recognize both isoforms, may also interact with chicken

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human XBP-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-XBP1 antibody (ABIN732728)

Li, Han, Shi: "IRE1?-XBP1 Pathway Is Activated Upon Induction of Single-Prolonged Stress in Rat Neurons of the Medial Prefrontal Cortex." in: Journal of molecular neuroscience : MN, (2015) (PubMed).

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1

Background:

Synonyms: XBP2, TREB5, XBP-1, X-box-binding protein 1, Tax-responsive element-binding protein 5, XBP1

Background: Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.

Gene ID: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response