Transferrin Receptor antibody (CF®405S)

Catalog No. ABIN7661020

This Mouse Monoclonal antibody specifically detects Transferrin Receptor in FACS. It exhibits reactivity toward Human.

Quick Overview for Transferrin Receptor antibody (CF®405S) (ABIN7661020)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS)

Clone: TFRC-1396

Product Details anti-Transferrin Receptor Antibody (CF®405S)

Purpose: CD71 / Transferrin Receptor (TFRC)(TFRC/1396), CF405S conjugate

Characteristics: Recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TFRC protein

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 0.5-2 μg/million cells, Immunofluorescence: 1-2 μg/mL, Immunohistochemistry Frozen, 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: PC3 cells. Human placenta or breast carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71

Background:

Synonyms: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Gene Symbol: TFRC

Tissue Expression: Microvascular endothelial cells

Molecular Weight: 85-95 kDa (monomer), 190 kDa (dimer)

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis